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The hypothesis implies that there is a blueprint made by specific enzymes that allow the addition or removal of methyl or acetyl groups to histones, or to identify domains on the histone to be epigenetically altered, ultimately altering gene expression
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After treatment, the cells were loaded with 5 mm CellROX Orange Reagent for 30 min at 37°C, washed with DPBS and immediately imaged on a fluorescence microscope (Olympus, Center Valley, PA)
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