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The liver antioxidant defence was investigated by monitoring the activation of nuclear factor erythroid 2-related factor 2 (Nrf2) by phosphorylation, and expression of Nrf2-regulated antioxidative enzyme haem oxygenase 1 (HO-1), and by measuring the activity of the copper-zinc superoxide dismutase (CuZnSOD), manganese superoxide dismutase (MnSOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), and glutathione S-transferase (GST), free sulfhydryl groups (SH) and glutathione (GSH) concentration, and the level of lipid peroxidation (thiobarbituric acid reactive substances, TBARS)
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