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Nevertheless, the kinetic data for our mutants and rate constants for ScGrx7 and PfGrx between 10 5 –10 6 M −1 s −1 are too slow for a diffusion-controlled reaction and point towards important short-lived enzyme–substrate interactions and rate-limiting re-orientations of the substrate at the enzyme surface

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Metabolic engineering of anthocyanin biosynthesis in Escherichia coli