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The fixed organoid cultures were blocked in 5% donkey serum (Millipore, Billerica, MA, USA) + 0.3% Triton-X-100/PBS, incubated overnight in 3% bovine serum albumin (Sigma-Aldrich, St Louis, MO) + PBS with primary antibodies, washed, incubated with Alexa Fluor-conjugated secondary antibodies (Invitrogen, Carlsbad, CA), washed, and stained with DAPI or mounted in VECTASHIELD H-1000
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This symptomatic approach leaves the root cause of the illness—the ongoing exposure and accumulated toxins—untreated, allowing the patient's health to continue to decline
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