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The samples were neutralized after heating (60 min, 100 °C) with methanol/NaOH, centrifuged (3 min, 3000 rpm) and MDA was measured with high-performance liquid chromatography (HPLC) (excitation: λ 532 nm, emission: λ 563 nm, LaChrom Merck Hitachi Chromatography System, Vienna, Austria