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Tandem MS spectra for both positive and negative mode used a resolution of 15,000, AGC target of 1 × 10 5 , maximum IT of 50 ms, isolation window of 0.4 m / z , isolation offset of 0.1 m / z , fixed first mass of 50 m / z and three-way multiplexed normalized collision energies of 10, 35 and 80
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