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These effects appear to be more pronounced in aged than in younger subjects (n = 21, age 48–75) (Mahdi et al., 2019), and does not depend on efficacy of blood glucose-lowering therapy in T2D patients (n = 16, average age 64) (Mahdi et al., 2018)

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In the dynamic simulation of GSH_a, the conformation exhibiting a hydrogen bond between N259 and the ligand occurs 100% of the time, while E255 appears over 95% (Table 3)