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injection of lipopolysaccharides (LPS, 20 μg/kg, 93572-42-0, Sigma-Aldrich) was given for the first 3 days, pre-treatment with LPS creates a pro-inflammatory, pro-thrombotic, and hyperlipidemic state that significantly lowers the threshold for high-dose steroids to induce the vascular compromise and cellular damage characteristic of osteonecrosis in the rat femoral head
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