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The downstream effects of the two enzymes are opposite: ACE activity leads to vasoconstriction, oxidative stress, inflammation and apoptosis, whereas ACE2 causes vasodilatation, angiogenesis and anti-inflammatory, anti-oxidative and anti-apoptotic effects [1]

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Cell lysis was performed by sonication for 5 min in ice with alternating pulses of 0.4 s with intervals of 0.4 s (Sonoplus sonifier/MS73 sonotrode)