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Primary endpoints: tolerability, pharmacokinetics (Cmax, Tmax, AUC, half-life), and target engagement biomarkers (plasma 1-methylnicotinamide as surrogate for NNMT activity, whole-blood NAD+)
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Then 2 mL of 2M hydrochloric acid was added and extracted with 3 x 2 mL diethyl ether
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