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Cells were washed three times with 1 ml PBS and lysed with 200 µl 0.5% (v/v) Triton X-100 (Fisher Scientific) at 37 °C for 5 min, followed by the addition of 800 µl PBS

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Persistently elevated IGF-1 has been associated with increased risk of certain cancers in epidemiological studies (Renehan et al., Lancet 2004)

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