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Each sample was then split into three fractions (100 μL) and treated as follows: For H 2 S measurement, 100 μL sample was precipitated with 1 mL of absolute MeOH at –40 ° C for 2 h, centrifuged (16,000 × g ), and obtained supernatant was evaporated and the residue was resuspended and sonicated in 100 μL of 50 mM HEPES buffer (pH 7.4)

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6 Hemolysis occurs after exposure to the stressor but does not continue despite continued infection or ingestion