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Membranes were blocked with 5% BSA for one hour at room temperature, and then incubated with either a 1:1000 dilution of anti-GPX4 (rabbit) polyclonal Ab (Abcam, ab41787) or 1:5000 dilution of anti-β-Actin (mouse) mAb (Sigma, A5441) at 4°C overnight
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