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RBC membranes contain high levels of polyunsaturated fatty acids, which make them particularly vulnerable to peroxidation, causing a loss of membrane integrity and decreased activity of membrane enzymes like ATPase and acetylcholinesterase [56,57]

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A 0.5 ml portion of each assay preparation was added to a well in a 24-well plate, and they were incubated at 37 °C in 5% CO 2 for 1 h