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The CPZ-treated group exhibited marked reduction in the intensity of the LFB stain of the CC, and most fibers appeared disorganized and pale in color

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Overall, as AD progresses from the asymptomatic stage to the symptomatic stage, the composition of EV proteins undergoes dynamic changes, which not only reflects potential neuro-pathological changes but also offers hope for the early diagnosis of AD, monitoring of disease progression, and the development of targeted therapies