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The reduction in triple negative breast cell viability may depend on HT acting as a copper chelator, thus perturbating copper homeostasis, as demonstrated in MDA-MB-231 cells by 100 μM HT-mediated increase in the copper chaperone for superoxide dismutase (CCS) after 48 h, and reduction in the subunit II of the complex IV of the mitochondrial respiratory chain cytochrome c oxidase (CcO) after 72 h
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This finding highlights its potential use as a vaccine adjuvant for infectious and viral diseases
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