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The autophagy-inhibitor bafilomycin A1 (100 nM) and the autophagy-inducer rapamycin (10 μM) were used as negative and positive controls, respectively
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Consistent with human observations, H2O2 also promoted FOXO4 nuclear translocation in TM3 cells, along with an increase in senescence markers p53, phospho-p53, and p21, while reducing testosterone synthesis-related enzymes like CYP11A1 and CYP17A1
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