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The increased glucose (25 mM) was used to inhibit α-cell glucagon secretion to diminish glucagon levels to basal levels to improve the dynamic range of the assay and thus effectively unmask the role of ligand-mediated glucagon secretion as described earlier 45
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These results directly confirm that excessive NNMT activation suppresses NAD+ synthesis and impairs energy metabolism, while NNMT inhibition effectively reverses this process, establishing NNMT as a negative regulator of energy metabolism
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