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Cells were then stained with hematoxylin (DAKO, Mayer’s Hematoxylin, S3309) for 30 s, washed in ammonia for 30 s and then washed in dH 2 O

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For example, an occupational therapist may help individuals with ASD by: Leading activities that help improve attention and awareness (when the individual is theorized to be "under-reactive") or reduce overall arousal (in the case of "hyper-arousal")