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The assay was completed in PBS (pH 7.4) as assay buffer with 0.1% BSA and 0.01% Tween-20 added to reduce nonspecific interactions and 2% DMSO added to increase compound solubility

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SGLT2 inhibitors for primary and secondary prevention of cardiovascular and renal outcomes in type 2 diabetes: a systematic review and meta-analysis of cardiovascular outcome trials