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8th edition) Cells have defense system to prevent free radical injury Normally free radicals are neutralized by anti – oxidant enzymes and substances like Superoxide dismutase – present in cytoplasm and produced by mitochondria Catalase – produced by peroxisomes Glutathione peroxidase – produced by mitochondria and present in cytoplasm Vitamin C in cytoplasm Ferritin and Ceruloplasmin present in cytoplasm – Levels of active forms of iron and copper are minimized by binding of ions to storage and transport proteins like transferrin, ferritin, ceruloplasmin there by minimizing the formation of hydroxyl ion Vitamins E, A and β Carotene in the plasma membrane and cell membrane of organelles An imbalance between free radical generating & radical scavenging systems results in “oxidative stress” Fenton reaction Oxygen is converted into superoxide (O 2 – ) by oxidative enzymes in the endoplasmic reticulum, mitochondria, plasma membrane, peroxisomes and cytosol Superoxide (O 2 – ) is converted into Hydrogen peroxide by dismutation

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