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Methods EV-F3 production and purification BHK-21 cells were grown to 80% confluency before infection with EV-F3 virus stocks in Glasgow modified Eagle’s medium (G-MEM BHK-21, Gibco), supplemented with 2% fetal horse serum (Thermo Fisher Scientific) and minimal essential medium non-essential amino acids (MEM NEAA, Gibco)
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