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The reversible covalent adduct of odanacatib bound to recombinant cysteine protease CatK [82] can be detected by top-down MS analysis (Figure 3A), provided that inhibitor concentration exceeds its steady-state equilibrium constant K i * to ensure sufficient covalent occupancy [60]
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Characterization of disulfide bond rebridged fab–drug conjugates prepared using a dual maleimide pyrrolobenzodiazepine cytotoxic payload
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