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We believe that further studies will be needed to address this very interesting aspect of thymosin biology, such as the effects of Tβ4 on MS patients, and that of Tα1 on the EAE model, having in mind that treating MS patients with Tα1 and Tβ4 alone or together with other approved drugs may meet the requirements for a desirable but-still-unknown therapy for MS acting on both immune dysregulation and CNS damage
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Following the blocking step, the cells were further incubated with specific antibodies targeting iNOS (Lot: 17-5920-82, Thermo Fisher, USA) and CD206 (Lot: 17-2061-82, Thermo Fisher, USA) in 1% BSA buffer for an additional 30 minutes