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Then, 10 μg of each supernatant was mixed with 200 μl reaction buffer (50 mM Tris-HCl [pH 8.0], 2 mM β-mercaptoethanol, 1 mM MgCl 2 , 0.1% bovine serum albumin, 2 mM p-nitrophenylphosphate, and 74.07 μM dUTP [GeneAct] and 1.0 μCi [ 3 H]dUTP [Moravek, Inc.])
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