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The optimized mixture for both assays is comprised of 10 μl of TaqMan Fast Advanced Master Mix, 10 pmol of primers (IS900Forward or F57Forward and IS900Reverse or F57Reverse), 1 pmol each of the FAM-labeled IS900qPCR or F57qPCR probe and the Cy5-labeled IACqPCR probe, 0.2 U of Uracil DNA Glycosylase, 50 copies of the IS900 or F57 internal amplification control plasmids, and 4 μl of DNA sample, making a total volume of 20 μl (Slana et al., 2008)

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Splenic macrophages and dendritic cells grab the microorganisms and microbial products in the blood and then stimulate the T and B cells that arrive in the spleen from the blood