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Fluorescence in situ hybridisation microscopy Fluorescence in situ hybridisation (FISH) was carried out with a DCMF-specific oligonucleotide probe (Dcm623, 5′-/Cy3/CTCAAGTGCCATCTCCGA-3′), designed using ARB [29], and probe Eub338i (5′-/6-FAM/GCTGCCTCCCGTAGGAGT-3′) [30] to target all bacteria