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Following the incubation of 100 μL of each sample with 100 μL of Detection Solution A, 100 μL of Detection Solution B, 90 μL of substrate solution, and 50 μL of stop solution, a spectrometer (Infinite 200 Pro MPlex, Tecan, Männedorf, Switzerland) was used to measure the absorbance at 450 nm
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Cyclobutyl tridecyl phthalate forms conventional hydrogen bonds with Gly50, Lys35, and Gln51, significantly stabilizing it within the active site via enhanced molecular affinity
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