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DT40 cells—from KJ Patel’s laboratory stock—were grown in RPMI medium supplemented with 3 % chicken serum (Gibco, # 16010167), 7 % Fetal calf serum (Gibco, # 16110082), 50 μmol L −1 β-mercaptoethanol, and P/S 11

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The accumulation of these structures is consistent with a failure or overwhelming of the autophagy pathway