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To quantify the ciliary basal signal of phospho-(T94)Tctex-1, the total fluorescence of the region of interest, that is, the proximal end of Ac-Tub–labeled cilium, was quantified in the projection confocal images of 1.25-μm-thick Z-series sections of cilium-containing regions ( x = 1 μm, y = 1 μm) in randomly selected cells using NIH ImageJ 64 software
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Mitochondrial fusion is increased by the nuclear coactivator PGC-1beta
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