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Western blotting Cells from culture dishes were rinsed with cold 1×PBS and extracted with a lysis buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% (w/v) SDS, 2 mM sodium orthovanadate, 1 mM PMSF, Protease Inhibitor Cocktail (APExBIO, K1007), Phosphatase Inhibitor Cocktail (APExBIO, K1015))

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Regulation of Chondrocyte Gene Expression One of Cartalax’s most important effects is its influence on gene expression within cartilage cells