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Step 6*: A faster version of DIA-MS analysis using a classic DIA-MS method [TIMING ~2 h] [TIP] Alternatively, a regular DIA method of 1.5 or 2 h also can be used for the phosphoproteome data acquisition (Gao et al

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This aberrant activation promotes excessive phosphorylation at the Ser396, Ser404, and Thr231 sites of tau, ultimately driving its pathological aggregation [138]