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Potential measurements include: Cell-count changes DNA-synthesis assays Cell-cycle phase distribution Metabolic viability assays Colony formation Apoptosis-associated markers Membrane-integrity measurements Senescence-associated markers Increased proliferation is not automatically beneficial and does not establish tissue regeneration

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4.1 Effects on the male reproductive system Melatonin significantly improves the status of the male reproductive system, acting through the hypothalamic–pituitary–gonadal (HPG) axis to regulate the secretion of reproductive hormones, primarily GnRH and luteinizing hormone (LH) (71)