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Since the enzyme catalyzing the first reaction in a pathway is often the regulatory enzyme, this prevents: the synthesis of unneeded intermediates that would occur if regulation happened downstream

L Glutathione

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Residues forming the binding-pocket of mesophilic GGTs are mostly conserved, except differences of one or two residues, suggesting rigidity of the binding pocket: in EcGGT, Asn411 and Gln430 while Glu423 and Glu442 in BsGGT