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The figure below illustrates how the charge on a diprotic species (corresponding to an amino acid with an unionizable R group) varies with the "ambient" pH of the medium of which it is a component

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Frequently the model is used in parallel to EAE or other animal models to study treatment effects of substances that promote remyelination, such as benztropine, quentiapine fumarate, or antagonists of LINGO-1 (Mi et al., 2009