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Their structure is composed of four domains (Figure 2) [26, 30, 46, 47]: An amino-terminal arm An anti-parallel eight-stranded β-barrel domain Wrapping domain α-helical domain The amino-terminal domain is an extended arm and is quite variable in length ranging from 53 residues in catalase (PMC) to 127 in HPII [30, 47]

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The right volume depends on the total peptide mass listed on that specific vial, the total reconstituted volume a given research protocol calls for, and the resulting concentration that protocol requires three variables that differ across vial sizes, blends, and individual research designs