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Mammalian Cell Culture THP-1 cell line (ATCC, TIB-202, Manassas, VA, USA) was maintained at a cell density of 2 × 10 5 –8 × 10 5 mL in RPMI 1640 medium (Lonza, Bornem, Belgium) supplemented with 10% heat inactivated fetal bovine serum (GIBCO, Life Technologies, Waltham, MA, USA), 1× antibiotic-antimycotic solution (GIBCO, Life Technologies), 1 × glutamax (GIBCO, Life Technologies) in humidified 5% CO 2 atmosphere at 37 °C
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Redox signaling via the molecular chaperone BiP protects cells against endoplasmic reticulum-derived oxidative stress