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Then, 20 µL of MTT solutions was added to each well followed by an incubation period of 3 hours, then the media were removed and 200 µl of DMSO was added to dissolve the crystal and the absorbance was recorded at 570 nm (BioTek, USA) and cell viability was calculated as a percentage compared to cells treated with DMEM alone

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Potential tear biomarkers for the diagnosis of Parkinson’s disease—a pilot study