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5 Conclusion In summary, the addition of 0.75 μM Vit A and 2 μM Lup to bucks semen cryopreservation extenders significantly improved post-thawing sperm motility parameters, plasma membrane integrity, and antioxidant capacity, while reducing the proportion of apoptotic spermatozoa and may improve fertilization and early embryonic development in vitro , although further studies are needed to confirm this effect