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To generate mito GCL (E103A), the forward and reverse oligonucleotides 5′-CAGAGTATGGGAGTTACATGATT GCA GGGACACCTGGCCAGCCGTACGGA-3′ (mutant nucleotides underlined) were used

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The binding of a ligand to a TLR causes adaptor molecules–such as myeloid differentiation primary response 88 (MyD88), Toll/IL-1 receptor (TIR) domain-containing adaptor protein (TIRAP), TIR domain-containing adaptor-inducing interferon-β (TRIF), and TRIF-related adaptor molecule (TRAM)–to bind the TIR domain of the receptor