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This reaction demonstrates how antioxidants, including polyphenols, act as electron or hydrogen atom donors for free radicals, preventing their harmful effects on cellular components and halting the chain reactions of oxidative stress

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blots were incubated with rabbit primary antibody anti-Id2 (c-20) (Santa Cruz Biotechnology, CA, USA), diluted 1∶750 in 1% (w/v) non-fat dry milk TBS-Tween (TTBS) overnight at 4°C, or with α tubulin (Santa Cruz Biotechnology, CA, USA), diluted 1∶1000 in 1% (w/v) non-fat dry milk TTBS overnight at 4°C with shaking