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The filtered reads were mapping to the reference genome using HISAT2 v2.0.5.Then, the quality of the library was evaluated by an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, United States).After RNA extraction, purification, and library construction of the samples, next-generation sequencing technology was used to perform paired-end (PE) testing on these libraries using the Illumina sequencing platform (NEB, United States) 14
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