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Polymerase chain reaction (PCR) for TNF-α, IL-6, p16 and 18 s RNA (as an internal control) was also performed in duplicate using the following instruction and in a final volume of 20 μL: (1) 10 μL BIOFACT™ 2X Real-Time PCR Mix Master (for SYBR Green I
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doi: 10.1016/j.gendis.2016.04.007
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